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Multiplexing

 

 

 

your need:

  • multiplex design from your monoplex (SYBR / probe) assays
  • smart design in routine 4- to 6-plex, including internal control
  • smart primer/probe strategies for increased multiplexing up
  • to 100 targets per reaction)
    e.g. sloppy molecular beacons

 

 

Additional reading on sloppy molecular beacon probes:
Use of Sloppy Molecular Beacon Probes for Identification of Mycobacterial Species. El-Hajj et al., J. Clin. Microbiol. 2009: (47) 1190-1198

 

Rapid Universal Identifcation of Bacterial Pathogens from Clinical Cultures by Using a Novel Sloppy Molecular Beacon Melting Temperature Signature Technique. Chakravorty et al., J. Clin. Microbiol. 2010: (48) 258-267