your guide in qPCR

Fluorophore

Alternative Fluorophore

Excitation
(nm)

Emission
(nm)

Coumarin

Biosearch Blue ^A, LC Cyan 500 ^B

430

475

FAM

SYBR Green I

495

515

TET

CAL Fluor Gold 540 ^A

525

540

HEX

ATTO 532 ^C, CAL Fluor Orange 560 ^A, JOE, VIC ^D,
Yakima Yellow ^E

535

555

Cy3 ^F

NED ^D, Oyster 556 ^G, Quasar 570 ^A

550

570

TMR

Alexa 546 ^H, CAL Fluor Red 590 ^A

555

575

ROX

Alexa 568 ^H, CAL Fluor Red 610 ^A, LC Red 610 ^B, Redmond Red ^E

575

605

Texas Red

Alexa 594 ^H, CAL Fluor Red 610 ^A, LC Red 610 ^B, Redmond Red ^E

585

605

LC Red 640 ^B

CAL Fluor Red 635 ^A

625

640

Cy5 ^F

ATTO 647N ^C, LC Red 670 ^B, Oyster 645 ^G, Quasar 670 ^A

650

670

LC Red 705 ^B

Cy5.5 ^F, Quasar 705 ^A

680

710

A) Biosearch Blue, CAL and Quasar fluorophores are available from Biosearch Technologies

B) LC (Light Cycler) fluorophores are available from Roche Applied Science

C) ATTO dyes are available from ATTO-TEC

D) VIC and NED fluorophores are available from Applied Biosystems

E) Yakima Yellow and Redmond Red dyes are available from Epoch Biosciences

F) Cy dyes are available from Amersham Biosciences

G) Oyster fluorophores are available from Integrated DNA Technologies

H) Alexa fluorophores are available from Invitrogen